LYM
MON
NEU
EOS
Forward scatter
+
Side scatter
2
D
In 2D scattergram, the cell clusters appear close to each
other. Whereas with the 3D scattergram, clusters are well
separated which helps in detecting abnormal cells, if any.
Forward scatter
+
Side scatter
+
Fluorescence
3
D
LYM
MON
NEU
EOS
Small Cube, Big Difference