ZENIT•AMiDot slides can be run either manually or on automated IFA slide processors
The principal steps of the method are:
Serum sample dilution (1/80) and incubation with autoimmune antigen array fixed on glass slides for 30 min in a moisture chamber at RT
Incubation with flurophore conjugated goat anti-human IgG/IgA for 30 min at RT
Assessment: automated analysis of slides
Signal intensity is directly proportional to the amount of antibody present in the sample. Results are expressed in U/ml or IU/ml.
10 core values have influenced the progress of our group.